AbNano® Anti-T-Cell VHH Library
Introducing the AbNano Anti-T-Cell VHH library, a first in-class immune-derived single domain antibody library designed specifically to overcome the complexities of t-cell surface protein targeting.
Leveraging advanced immunization strategies and error-prone PCR diversification, this unique platform contains nearly 1 billion unique VHH molecules to rapidly identify novel antibody candidates.
Anti-T-Cell Library Technical Overview
The AbNano VHH T-Cell library begins with immunizing llamas using unstimulated human CD3+ T-cells, capturing a broad spectrum of native epitopes.
Initial sequencing revealed a diversity of approximately one million unique VHH sequences, which were subsequently expanded through serial error-prone PCR, achieving a final diversity nearing one billion unique protein-level VHH sequences.
- High Diversity & High Affinity: With nearly one billion diverse VHH molecules, AbNano enable rapid discovery of nanomolar-affinity binders
- Unique Targeting Strategies: Immune-driven diversity ensures relevance and specificity in antibody identification
- Versatile Platform: Ideal for bioprocess optimization, diagnostics, and early therapeutic discovery
What Separates Natural Libraries from Synthetic?
Unlike synthetic libraries, AbNano VHH libraries deliver immune-driven diversity for more relevant antibody discovery. Our modular workflow is adaptable for future antibody campaigns in immunology and immuno-oncology.
Validated Targets & Affinity Data
- CD3ε: 34 unique clonotypes identified from 116 hits using ELISA screening.
- CD8α/CD8β: 43 unique clonotypes identified from 58 hits.
- Binding affinities range from high nanomolar to low micromolar, ensuring robust interaction profiles suitable for a wide range of research applications.
Streamline Your Discovery Timelines
Reduce late-stage attrition and streamline discovery timelines by starting your antibody campaigns with our naturally derived, validated VHH library. Whether you’re supporting a technical team or evaluating innovative solutions at a strategic level, the AbNano platform is engineered to deliver impactful results swiftly and reliably.
Choose the workflow that best suits your project’s requirements:
Solid-Phase Panning
Rapid, precise identification of VHH binders using recombinant protein targets adsorbed onto styrene plates.
Solution-Phase Panning
Enhanced flexibility with biotinylated targets captured via streptavidin beads, ideal for native-like binding conditions.
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