AbNano™ Anti-T-Cell VHH Library

Introducing the AbNano™ Anti-T-Cell VHH library, a first in-class immune-derived single domain antibody library designed specifically to overcome the complexities of t-cell surface protein targeting.

Leveraging advanced immunization strategies and error-prone PCR diversification, this unique platform contains nearly 1 billion unique VHH molecules to rapidly identify novel antibody candidates.

Anti-T-Cell Library Technical Overview

Diagram of camelid immunization and VHH nanobody library generation workflow from antigen design to phage display screening.

The AbNano VHH T-Cell library begins with immunizing llamas using unstimulated human CD3+ T-cells, capturing a broad spectrum of native epitopes. Initial sequencing revealed a diversity of approximately one million unique VHH sequences, which were subsequently expanded through serial error-prone PCR, achieving a final diversity nearing one billion unique protein-level VHH sequences.

Key Benefits & Applications:

High Diversity & High Affinity: With nearly one billion diverse VHH molecules, AbNano™ enable rapid discovery of nanomolar-affinity binders
Unique Targeting Strategies: Immune-driven diversity ensures relevance and specificity in antibody identification
Versatile Platform: Ideal for bioprocess optimization, diagnostics, and early therapeutic discovery

50% off Anti-Cell-Libraries until 3.31.26

Use code VHH26Q1 on Your Quote >

What Separates Natural Libraries from Synthetic?

Unlike synthetic libraries, AbNano VHH libraries deliver immune-driven diversity for more relevant antibody discovery. Our modular workflow is adaptable for future antibody campaigns in immunology and immuno-oncology.

See how this approach can accelerate your research.

Download The Poster >

Scientific poster on nanomolar VHH binders to PD-L1 discovered using the AbNano™ VHH naive library, showing screening data and results.

Validated Targets & Affinity Data

CD3ε: 34 unique clonotypes identified from 116 hits using ELISA screening.
CD8α/CD8β: 43 unique clonotypes identified from 58 hits.
Binding affinities range from high nanomolar to low micromolar, ensuring robust interaction profiles suitable for a wide range of research applications.

Bar chart of clonal off-phage ELISA screening of Anti-T-Cell VHH library against CD3e showing binding signal across clones.

Bar chart of clonal off-phage ELISA screening of Anti-T-Cell VHH library against CD8a/CD8b showing binding signal across clones.

Figures: Clonal crude VHH were prepared by osmotic shock and assayed against recombinant CD3ε or CD8α/CD8β on streptavidin-coated plates. The single-point binding was detected by HRP-anti-VHH conjugate and ABTS.

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Streamline Your Discovery Timelines

Reduce late-stage attrition and streamline discovery timelines by starting your antibody campaigns with our naturally derived, validated VHH library. Whether you’re supporting a technical team or evaluating innovative solutions at a strategic level, the AbNano™ platform is engineered to deliver impactful results swiftly and reliably.

Choose the workflow that best suits your project’s requirements: