AbNano™ VHH Anti-T-Cell Library Data Pack
Accelerate your T-cell target discovery with a
semi-synthetic, immunization-derived VHH library built for phage display.
Constructed from an immunized llama challenged with human CD3+ T-cells and
diversified by error-prone PCR, this library delivers an estimated 7 × 108 unique
clonal VHH displayed on monovalent phage. Access the full data pack to review
detailed sequencing QC, validated panning results against CD3 epsilon and CD8
alpha/beta, and practical guidance for integrating the library into your
existing phage display workflows.
What You Get Inside
Product overview of the AbNano VHH Anti-T-Cell Library: Construction from an immunized llama using human CD3+ T-cells as the immunogen, diversification by error-prone PCR across four sublibraries, and delivery as phage-displayed, monovalent VHH (catalog # L760-100).
Detailed sequencing QC: V-gene usage (99.98% IGHV3), CDR3 length distribution, cluster density and frequency analysis, and position-specific weight matrices for CDR1, CDR2, and CDR3 — all generated from 1.31 million long-read NGS reads.
Application data: Representative panning campaigns against CD3 epsilon and CD8 alpha/beta heterodimer, including clonal screening hit rates, unique binder counts, and EC50 binding curves with affinities in the nanomolar range.
Practical product information: Physical characteristics (titer, volume, buffer, storage), phagemid backbone (pADL-20c), representative VHH framework map, and sequencing primer locations for NGS and Sanger workflows.
Who Would Benefit
- Antibody discovery teams pursuing VHH
binders against T-cell surface markers for therapeutic, diagnostic, or research
applications, including phage display, CAR-T, and bispecific formats.
- Scientists evaluating immunized versus naïve or synthetic VHH library strategies and seeking validated panning data on clinically relevant T-cell targets such as CD3 and CD8.
- Biotech and pharma groups needing a
well-characterized, immunization-derived starting library with documented QC,
sequencing data, and demonstrated nanomolar-affinity binder output.
Related Resources
- [Product] AbNano VHH Anti-T-Cell Library Product Details
- [Service] Custom VHH Discovery
- [Scientific Poster] An Anti-T-Cell Immunization Strategy for a Novel, Targeted Immune-Derived Library
