Generation and validation of a recombinant monoclonal antibody to PAX8: A multi-assay approach

Paired box 8 (PAX8) is a nuclear protein involved in thyroid follicular cell development and expression of thyroid-specific genes. While primarily associated with thyroid follicular carcinomas and atypical follicular thyroid adenomas, recent research has indicated that this marker is prognostic in small-cell lung cancer and positively correlated with Ki67 expression (Tao et al., 2024) -- perhaps opening PAX8 up as a future therapeutic target. Studies such as these, though, require the use of extremely reliable antibodies to generate reproducible and trustworthy data.

In congruence with the International Working Group for Antibody Validation’s (IWGAV) pillars of antibody validation, a program has been implemented to enhance our existing validation principles including the recombinant monoclonal PAX8.