Scientists purchase antibodies with the expectation that they will recognize their intended targets and produce reliable data, but the presence of improperly validated antibodies on the market can make this process both time-consuming and expensive. Target recognition and specificity is fundamental to ensuring the rigor and reproducibility of data generated by immunoblotting, immunohistochemistry, ELISA, and other assays that rely on antibodies.
Bethyl antibodies are highly regarded for passing strict validation testing before arriving in customers’ hands. The principles that have guided our rigorous validation practices for decades preceded a recent publication* that describes five conceptual pillars for validating antibodies in an application- and context-specific manner.
* Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T. A proposal for validation of antibodies. Nat Methods. 2016 Oct;13(10):823-7. PMID: 27595404.