Bethyl Laboratories
Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H]
Monoclonal Antibodies
Catalog #A700-101
TargetCDK1 Phospho (Y15)
HostRabbit
CloneBLR101H
ReactivityHuman
ApplicationIP, WB
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Product Description
Cyclin-dependent kinase 1 (CDK1) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CKD1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control [taken from NCBI Entrez Gene (Gene ID: 983)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR101H
Host
Rabbit
Immunogen
Surrounding tyrosine 15
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
40 µg/ml
Target Identity
TargetCDK1 Phospho (Y15)
Antigen Species
Human
Gene ID983
Gene Symbol
CDK1
Gene Name
cyclin dependent kinase 1
Uniprot IDP06493
Protein Name
Cyclin-dependent kinase 1
Product Information
Applications
IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide surrounding phosphorylated tyrosine 15 of human cyclin dependent kinase 1 using the numbering given in entry NP_001777.1 (Gene ID 983).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-101 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-101 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-101 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-101Open
Services
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