Bethyl Laboratories
Rabbit anti-AHR Recombinant Monoclonal Antibody [BLR118H]
Monoclonal Antibodies
Catalog #A700-118
TargetAHR
HostRabbit
CloneBLR118H
ReactivityHuman
ApplicationICC, IHC, IP, WB
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Product Description
AHR is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, AHR is sequestered in the cytoplasm; upon ligand binding, the protein moves to the nucleus and stimulates transcription of target genes. [taken from NCBI Entrez Gene (Gene ID: 196)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR118H
Host
Rabbit
Immunogen
Between 798 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetAHR
Antigen Species
Human
Gene ID196
Gene Symbol
AHR
Gene Name
aryl hydrocarbon receptor
Uniprot IDP35869
Protein Name
Aryl hydrocarbon receptor
Alternate Names
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 798 and the C-terminus (residue 848) of human aryl hydrocarbon receptor using the numbering given in entry NP_001612.1 (Gene ID 196).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-118 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-118 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-118 · Lot 200429Open
Safety Data SheetPDF · A700-118Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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