Bethyl Laboratories
ELISA Blocking Buffer
ELISA Accessories
Catalog #E104
ApplicationELISA
Product Description
Required for blocking unbound portions of ELISA wells to minimize non-specific binding that can occur with the secondary antibody prior to development.
Specifications
Product Information
Applications
ELISA
Storage Conditions
2 - 8 °C
Shelf Life
1 year from date of receipt
Contents
Tris Buffered Saline with 1% BSA, pH 8.0. Sigma Chemical #T-6789, 1 packet (makes 1 liter)
Country of Origin
USA
Applications
Prepared Tris buffered saline with 1% BSA, pH 8.0, is suitable for use as a postcoat solution in ELISA.
Prepared Tris buffered saline with 1% BSA, pH 8.0, plus 0.05% Tween 20 (0.5 ml of 10% Tween 20 per 100 ml of postcoat) is suitable for use as sample and conjugate diluent.
Enzyme-Linked Immunosorbent Assay (ELISA)Dissolve contents of one packet in one liter deionized water to yield a 0.05 M Tris, 0.138 M NaCl, 0.0027 M KCl, pH 8.0 at 25 oC, with 1% BSA.
Documents
Product DatasheetPDF · E104 · Lot 0 (current lot)Open
Safety Data SheetPDF · E104Open
Citations / Publications
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