Abstract: The high-throughput screening system illustrated here produced a UBQLN4 recombinant monoclonal antibody capable of binding to its target protein with high specificity and affinity. This specificity was judged by utilizing an adapted "pillar" system and we utilized the pillars for "independent antibodies," "complementary assays," and "genetic strategies." Given this specificity, we were also able to show utility in other assays, such as flow cytometry and a 6-plex multiplex IHC assay. This antibody is well suited to continue studying the role of UBQLN4 in DNA damage response and its relationship with other proteins in this pathway.
