Bethyl Laboratories
Goat anti-Rabbit IgG Heavy and Light Chain Highly Cross-Adsorbed Antibody FITC Conjugated
Secondary
Catalog #A120-501F
TargetRabbit IgG Heavy and Light Chain
HostGoat
ClonalityPolyclonal
ReactivityRabbit
ApplicationFlow Cyt, ICC, IF, IHC
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Product Description
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Rabbit
Format
Whole IgG
Conjugate Type
FITC
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
Flow Cyt, ICC, IF, IHC
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
This antibody reacts specifically with rabbit IgG in immunohistochemistry and immunocytochemistry fluorescence assays. Non-specific binding to human and mouse tissue is negligible.
Applications
Not all listed applications have been specifically tested by our laboratory.
Flow Cytometry (Flow Cyt)1:50 - 1:200
Immunocytochemistry (ICC)1:100 - 1:500
Immunofluorescence (IF)1:100 - 1:500. For use with cells
Immunohistochemistry (IHC)1:100 - 1:500. For use with Formalin-Fixed Parrafin-Embedded Tissues
Documents
Safety Data SheetPDF · A120-501FOpen
Product DatasheetPDF · A120-501F · Lot 7 (current lot)Open
Safety Data SheetPDF · A120-501FOpen
Citations / Publications
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Resources
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