Bethyl Laboratories
Sheep anti-Human Lambda Free Light Chain Antibody IgG Fraction
Antisera
Catalog #A80-127
TargetHuman Lambda Free Light Chain
HostSheep
ClonalityPolyclonal
ReactivityHuman
ApplicationDD, IEP
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Product Description
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains - kappa and lambda. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. Antibodies against lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas.
Specifications
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
IgG Fraction
Product Information
Applications
DD, IEP
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
Total protein was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Total Protein: 15 mg/ml
By immunoelectrophoresis this antibody reacts specifically with lambda free light chain in Bence Jones Urines. No antibody was detected against kappa light chains or serum proteins.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP), double diffusion Ouchterlony (DD) or immunofixation electrophoresis (IFE)).
Optimal working dilutions should be determined experimentally by the investigator.
Double Diffusion Ouchterlony (DD)Neat
Immunoelectrophoresis (IEP)Neat
Documents
Safety Data SheetPDF · A80-127Open
Product DatasheetPDF · A80-127 · Lot 4 (current lot)Open
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