Purified Human IgA normal serum

Specifications

Production & Epitope

Purified normal human IgA is isolated using fractionation and specific affinity chromatography.

Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis the IgA was shown to 1) react with antiserum specific for human IgA, 2) not react with antiserum specific for IgG, IgM and 3) produce a single precipitin arc with antiserum against human serum identical to that produced with anti-IgA.

By ELISA the IgA contains less than 1% of the other immunoglobulin classes.

Applications

Used as a positive control in immunoassays.

Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for AntiHBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.

Used as a positive control in immunoassays.

Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for AntiHBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.