Purified Human IgG, kappa normal serum

Specifications

Production & Epitope

Purified normal human IgG is isolated using fractionation and DEAE chromatography. IgG with kappa chains was purified using affinity chromatography.

Protein concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis the IgG, kappa was shown to 1) react with antiserum specific for human IgG-Fc and kappa chains, 2) not react with antiserum specific for IgA, IgM or lambda chain and 3) produce a single precipitin arc with antiserum against human serum identical to that produced with anti-IgG.

By ELISA the IgG, kappa contains less than 1% of IgA, IgM and lambda.

Applications

Used as a positive control in immunoassays.

Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for AntiHBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.

Used as a positive control in immunoassays.

Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for AntiHBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.