Donkey anti-Human IgG Heavy and Light Chain Cross-Adsorbed Antibody FITC Conjugated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 680.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 800.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species. Antiserum was cross adsorbed using bovine, chicken, goat, mouse, rabbit, rat & sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 755.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, mouse, rabbit, rat & sheep IgG was detected. This antibody may cross react with IgG from other species.
Additional Product Information
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Applications
Not all listed applications have been specifically tested by our laboratory.
DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 680 is excited at 682 (in PBS) and emits at 715 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 800 is excited at 770 (in PBS) and emits at 794 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 755 is excited at 754 (in PBS) and emits at 776 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.