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Donkey anti-Sheep IgG Heavy and Light Chain Antibody Biotinylated

bethyl
Bethyl Laboratories Catalog #
Target:
Sheep IgG Heavy and Light Chain
Reactivity:
Sheep
Host:
Donkey
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin, DyLight® 488, DyLight® 550, DyLight® 594, DyLight® 650, FITC, HRP, Unconjugated
Purity:
Antigen Affinity Purified, Antiserum
Antiserum (2 ml)

Unconjugated (1 mg)

Biotin (1 mg)

DyLight® 488 (0.5 mg)

DyLight® 550 (0.5 mg)

DyLight® 594 (0.5 mg)

DyLight® 650 (0.5 mg)

FITC (1 mg)

HRP (1 mg)

$50.00 $129.00
Qty:

Product Details

Specifications

Verified Reactivity
Sheep
Antigen Species
Sheep
Concentration
1 mg/ml 0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt, 2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.05% Pro-Clean 400
Request Formulation Change Serum containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Biotin/antibody protein ratio is 4:1.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. Biotinylated antibody was demonstrated by reaction with avidin/peroxidase when coated on microtiter wells. Working dilutions should be determined by the investigator.
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
By immunoelectrophoresis, the antiserum reacts specifically with Sheep IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

This antibody may cross react with IgG from other species.
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 694.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Country of Origin
USA

Additional Product Information

Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.

Applications

Not all listed applications have been specifically tested by our laboratory. For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).

Optimal working dilutions should be determined experimentally by the investigator. DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.