Bethyl Laboratories
F(ab')2 Donkey anti-Rat IgG Heavy and Light Chain Antibody Affinity Purified
Secondary
Catalog #A110-142A
TargetRat IgG Heavy and Light Chain
HostDonkey
ClonalityPolyclonal
ReactivityRat
ApplicationELISA, ICC, IHC, WB
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Product Description
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Specifications
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Reactivity
Rat
Format
F(ab')2
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules were removed.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A110-142AOpen
Product DatasheetPDF · A110-142A · Lot 2 (current lot)Open
Safety Data SheetPDF · A110-142AOpen
Product DatasheetPDF · A110-142A · Lot 5Open
Safety Data SheetPDF · A110-142AOpen
Product DatasheetPDF · A110-142A · Lot 2 (current lot)Open
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