Goat anti-Dog IgA Heavy Chain Antibody HRP Conjugated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Serum containing 0.09% Sodium Azide
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By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgA. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgA. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgA. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity.
By immunoelectrophoresis, the antiserum reacts specifically with dog IgA. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgA from other species.
Additional Product Information
IgA is an immunoglobulin that is typically dimeric and held together by a J-chain protein. IgA is typically found near the mucous membranes of the body, including the gut, respiratory system, eyes, and colostrum. Also exists in a secretory form (in mucosal regions) vs. serum IgA, which is found in serum.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.