Goat anti-Dog IgG-Fc Fragment Antibody HRP Conjugated
Product Details
Specifications
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Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme:antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross-react with IgG from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross-react with IgG from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross-react with IgG from other species.
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Applications
Not all listed applications have been specifically tested by our laboratory.