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Goat anti-Donkey IgG Heavy and Light Chain Antibody Biotinylated

bethyl
Bethyl Laboratories

Catalog #

Target:

Donkey IgG Heavy and Light Chain

Reactivity:

Donkey

Host:

Goat

Clonality:

Polyclonal

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Biotin

,

DyLight® 488

,

DyLight® 550

,

DyLight® 594

,

DyLight® 650

,

FITC

,

HRP

,

Unconjugated

Purity:

Antigen Affinity Purified

Unconjugated (1 mg)

Biotin (1 mg)

DyLight® 488 (0.5 mg)

DyLight® 550 (0.5 mg)

DyLight® 594 (0.5 mg)

DyLight® 650 (0.5 mg)

FITC (1 mg)

HRP (1 mg)

Product Details

Specifications
Verified Reactivity

Donkey

Antigen Species

Donkey

Concentration
1 mg/ml 0.5 mg/ml
Fluorophore/Protein
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide


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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400


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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide


Request Formulation Change
Production Procedures

The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

Additional Product Information

Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.

Applications

Not all listed applications have been specifically tested by our laboratory. DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.