Bethyl Laboratories
Goat anti-Human IgE Heavy Chain Antibody Affinity Purified
Anti-Human Immunoglobulins
Catalog #A80-108A
TargetHuman IgE Heavy Chain
HostGoat
ClonalityPolyclonal
ReactivityHuman
ApplicationELISA, ICC, IHC, WB

Product Description
IgE monomers contain two heavy chains and two light chains, and are primarily involved in the immune response to parasites, and has been associated with allergies and asthma.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgE. Cross reactivity with other human immunoglobulins and light chains is less than 1%.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A80-108AOpen
Product DatasheetPDF · A80-108A · Lot 260629 (current lot)Open
Safety Data SheetPDF · A80-108AOpen
Product DatasheetPDF · A80-108A · Lot 22Open
Safety Data SheetPDF · A80-108AOpen
Product DatasheetPDF · A80-108A · Lot 14Open
Safety Data SheetPDF · A80-108AOpen
Citations / Publications
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