Bethyl Laboratories
Goat anti-Human IgM-Fc Fragment Antibody Affinity Purified
Anti-Human Immunoglobulins
Catalog #A80-100A
TargetHuman IgM-Fc
HostGoat
ClonalityPolyclonal
ReactivityHuman
ApplicationELISA, ICC, IHC, WB
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Product Description
IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgM. Cross reactivity with other immunoglobulins and light chains is less than 1%. This antibody may cross react with IgM from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A80-100AOpen
Product DatasheetPDF · A80-100A · Lot 260528 (current lot)Open
Safety Data SheetPDF · A80-100AOpen
Product DatasheetPDF · A80-100A · Lot 35Open
Safety Data SheetPDF · A80-100AOpen
Product DatasheetPDF · A80-100A · Lot 14Open
Safety Data SheetPDF · A80-100AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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