Bethyl Laboratories
Goat anti-Monkey IgG Heavy and Light Chain Antibody Cross-Adsorbed Affinity Purified
Secondary
Catalog #A140-202A
TargetMonkey IgG Heavy and Light Chain
HostGoat
ClonalityPolyclonal
ReactivityMonkey
ApplicationELISA, ICC, IHC, WB
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Product Description
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Monkey
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was absorbed against human serum, isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with monkey IgG and with light chains common to other monkey immunoglobulins. Species detected are Rhesus, and Cynomolgus. Reaction to human IgG was less than 1%. No antibody was detected against non-immunoglobulin serum proteins.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:20,000; for coating plates 1:50 - 1:250
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:20,000
Documents
Safety Data SheetPDF · A140-202AOpen
Product DatasheetPDF · A140-202A · Lot 260630 (current lot)Open
Safety Data SheetPDF · A140-202AOpen
Product DatasheetPDF · A140-202A · Lot 21Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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Simple Western™ Resources
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