Bethyl Laboratories
Goat anti-Rabbit IgG Heavy and Light Chain Cross-Adsorbed Antibody Affinity Purified
Secondary
Catalog #A120-201A
TargetRabbit IgG Heavy and Light Chain
HostGoat
ClonalityPolyclonal
ReactivityRabbit
ApplicationELISA, ICC, IHC, WB
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Product Description
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Rabbit
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:20,000; for coating plates 1:50 - 1:250
Immunocytochemistry (ICC)1:100 - 1:1000
Immunohistochemistry (IHC)1:100 - 1:1000
Western Blot (WB)1:1000 - 1:20,000
Documents
Safety Data SheetPDF · A120-201AOpen
Product DatasheetPDF · A120-201A · Lot 260416 (current lot)Open
Safety Data SheetPDF · A120-201AOpen
Product DatasheetPDF · A120-201A · Lot 21Open
Safety Data SheetPDF · A120-201AOpen
Product DatasheetPDF · A120-201A · Lot 13Open
Safety Data SheetPDF · A120-201AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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