Goat anti-Rat IgG+IgM+IgA Heavy and Light Chain Antibody HRP Conjugated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG, IgA and IgM and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG, IgA and IgM and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigens coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG, IgA and IgM and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma and functions to neutralize toxins and cell-mediated cytotoxicity. IgM is typically referred to as a pentamer, and is found primarily in serum and is the first part of the antibody response to infection. IgA is an immunoglobulin that is typically dimeric and held together by a J-chain protein. IgA is typically found near the mucous membranes of the body, including the gut, respiratory system, eyes, and colostrum.
Applications
Not all listed applications have been specifically tested by our laboratory.