Bethyl Laboratories
Goat anti-Rat IgG2b Heavy Chain Antibody Affinity Purified
Secondary
Catalog #A110-111A
TargetRat IgG2b Heavy Chain
HostGoat
ClonalityPolyclonal
ReactivityRat
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG2b structure is more restricted than the traditional Y-shaped IgG2a molecule. 2b tends to be shaped more like a T as a result of intramolecular disulfide interactions.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Rat
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG2b. This antibody may cross react with IgG2b from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A110-111AOpen
Product DatasheetPDF · A110-111A · Lot 241204 (current lot)Open
Safety Data SheetPDF · A110-111AOpen
Product DatasheetPDF · A110-111A · Lot 21Open
Safety Data SheetPDF · A110-111AOpen
Product DatasheetPDF · A110-111A · Lot 37Open
Services
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Antibody and conjugate formats developed together, built to your specification.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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