Bethyl Laboratories
Goat anti-Rat IgM Cross-Adsorbed Antibody Affinity Purified
Secondary
Catalog #A110-200A
TargetRat IgM Heavy Chain
HostGoat
ClonalityPolyclonal
ReactivityRat
ApplicationELISA, ICC, IHC, WB
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Product Description
IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Rat
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgM was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgM was detected. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:20,000; for coating plates 1:50 - 1:250
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:20,000
Documents
Safety Data SheetPDF · A110-200AOpen
Product DatasheetPDF · A110-200A · Lot 260126 (current lot)Open
Safety Data SheetPDF · A110-200AOpen
Product DatasheetPDF · A110-200A · Lot 7Open
Safety Data SheetPDF · A110-200AOpen
Product DatasheetPDF · A110-200A · Lot 15Open
Safety Data SheetPDF · A110-200AOpen
Product DatasheetPDF · A110-200A · Lot 12Open
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