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Rabbit anti-Bottlenose Dolphin IgG Heavy and Light Chain Antibody HRP Conjugated
Bethyl Laboratories
Catalog #
Target:
Bottlenose Dolphin IgG Heavy and Light Chain
Reactivity:
Bottlenose Dolphin
Applications:
ELISA,
ICC,
IHC,
WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Verified Reactivity
Bottlenose Dolphin
Antigen Species
Bottlenose Dolphin
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bottlenose dolphin IgG and with light chains common to other bottlenose dolphin immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bottlenose dolphin IgG and with light chains common to other bottlenose dolphin immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
This antibody may cross react with IgG from other species.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bottlenose dolphin IgG and with light chains common to other bottlenose dolphin immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bottlenose dolphin IgG and with light chains common to other bottlenose dolphin immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
This antibody may cross react with IgG from other species.
Country of Origin
USA
Additional Product Information
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Applications
Not all listed applications have been specifically tested by our laboratory.