Bethyl Laboratories
Rabbit anti-Goat IgG-Fc Fragment Cross-Adsorbed Antibody Affinity Purified
Secondary
Catalog #A50-204A
TargetGoat IgG-Fc Fragment
HostRabbit
ClonalityPolyclonal
ReactivityGoat
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Specifications
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Reactivity
Goat
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG-Fc Fragment. Less than 1% cross reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:20,000; for coating plates 1:50 - 1:250
Immunocytochemistry (ICC)1:100 - 1:1000
Immunohistochemistry (IHC)1:100 - 1:1000
Western Blot (WB)1:1000 - 1:20,000
Documents
Safety Data SheetPDF · A50-204AOpen
Product DatasheetPDF · A50-204A · Lot 5 (current lot)Open
Safety Data SheetPDF · A50-204AOpen
Product DatasheetPDF · A50-204A · Lot 5 (current lot)Open
Safety Data SheetPDF · A50-204AOpen
Product DatasheetPDF · A50-204A · Lot 5 (current lot)Open
Safety Data SheetPDF · A50-204AOpen
Product DatasheetPDF · A50-204A · Lot 2Open
Safety Data SheetPDF · A50-204AOpen
Product DatasheetPDF · A50-204A · Lot 3Open
Safety Data SheetPDF · A50-204AOpen
Product DatasheetPDF · A50-204A · Lot 6Open
Safety Data SheetPDF · A50-204AOpen
Product DatasheetPDF · A50-204A · Lot 6Open
Safety Data SheetPDF · A50-204AOpen
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