Bethyl Laboratories
Sheep anti-Bovine IgA Heavy Chain Antibody Affinity Purified
Secondary
Catalog #A10-131A
TargetBovine IgA Heavy Chain
HostSheep
ClonalityPolyclonal
ReactivityBovine
ApplicationELISA, ICC, IHC, WB
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Product Description
IgA is an immunoglobulin that is typically dimeric and held together by a J-chain protein. IgA is typically found near the mucous membranes of the body, including the gut, respiratory system, eyes, and colostrum. Also exists in a secretory form (in mucosal regions) vs. serum IgA, which is found in serum.
Specifications
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Reactivity
Bovine
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine IgA. This antibody may cross react with IgA from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A10-131AOpen
Product DatasheetPDF · A10-131A · Lot 260522 (current lot)Open
Safety Data SheetPDF · A10-131AOpen
Product DatasheetPDF · A10-131A · Lot 7Open
Safety Data SheetPDF · A10-131AOpen
Product DatasheetPDF · A10-131A · Lot 4Open
Safety Data SheetPDF · A10-131AOpen
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