Bethyl Laboratories
Sheep anti-Bovine IgG Heavy Chain Antibody Affinity purified
Secondary
Catalog #A10-118A
TargetBovine IgG Heavy Chain
HostSheep
ClonalityPolyclonal
ReactivityBovine
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain.
Specifications
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Reactivity
Bovine
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine IgG. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A10-118AOpen
Product DatasheetPDF · A10-118A · Lot 251113 (current lot)Open
Safety Data SheetPDF · A10-118AOpen
Product DatasheetPDF · A10-118A · Lot 15Open
Safety Data SheetPDF · A10-118AOpen
Product DatasheetPDF · A10-118A · Lot 12Open
Safety Data SheetPDF · A10-118AOpen
Product DatasheetPDF · A10-118A · Lot 19Open
Citations / Publications
Services
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