Bethyl Laboratories
Sheep anti-Dog IgG2 Heavy Chain Antibody Affinity Purified
Secondary
Catalog #A40-121A
TargetDog IgG2 Heavy Chain
HostSheep
ClonalityPolyclonal
ReactivityDog
ApplicationELISA, ICC, IHC, WB
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Product Description
IgG2 is the second most prominent subclass of IgG and does not cross the placenta easily but does bind strongly to Fc receptors. However, the half-life of IgG3 molecules is much shorter than IgG1.
Specifications
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Reactivity
Dog
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG2. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG2 from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A40-121AOpen
Product DatasheetPDF · A40-121A · Lot 260416 (current lot)Open
Safety Data SheetPDF · A40-121AOpen
Product DatasheetPDF · A40-121A · Lot 11Open
Safety Data SheetPDF · A40-121AOpen
Product DatasheetPDF · A40-121A · Lot 32Open
Citations / Publications
Services
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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