Bethyl Laboratories
Rabbit anti-CSN1 Antibody Affinity Purified
Primary
Catalog #A300-026A
TargetCSN1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
CSN1 is one of eight subunits (CSN1 to 8) of the highly conserved COP9 signalsome (CSN) complex originally identified as a regulator of light-mediated development in Arabidopsis. Characterization of CSN from yeast to mammals reveals a function as a modulator of signal transduction pathways involved in a variety of cellular and developmental processes. One of the major functions of the CSN is the regulation of protein degradation via intersection with the ubiquitin-proteasome pathway and regulation of E3-ubiquitin ligases.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 441 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCSN1
Antigen Species
Human
Gene ID2873
Gene Symbol
GPS1
Gene Name
G protein pathway suppressor 1
Uniprot IDQ13098
Protein Name
COP9 signalosome complex subunit 1
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CSN1 immobilized on solid support.
The epitope recognized by A300-026A maps to a region between residue 400 and the C-terminus (residue 471) of human G protein Pathway Suppressor 1 using the numbering given in Swiss-Prot entry Q13098 (GeneID 2873).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-026A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-026A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-026AOpen
Citations / Publications
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Resources
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