Bethyl Laboratories
Rabbit anti-Phospho SMC1 (S966) Antibody Affinity Purified
Primary
Catalog #A300-050A
TargetSMC1 Phospho (S966)
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC, IP, WB
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Product Description
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 966
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSMC1 Phospho (S966)
Antigen Species
Human
Gene ID8243
Gene Symbol
SMC1A
Gene Name
structural maintenance of chromosomes 1A
Uniprot IDQ14683
Protein Name
Structural maintenance of chromosomes protein 1A
Alternate Names
Product Information
Applications
ICC-IF, IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to phosphorylation of SMC1 at serine 966 immobilized on solid support.
Immunogen for A300-050A was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:500 - 1:5000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunohistochemistry (IHC)1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-050A · Lot 7 (current lot)Open
Product DatasheetPDF · A300-050A · Lot 7 (current lot)Open
Safety Data SheetPDF · A300-050AOpen
Citations / Publications
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