Bethyl Laboratories
Rabbit anti-SMARCA3 Antibody Affinity Purified
Primary
Catalog #A300-230A
TargetSMARCA3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA3 also known as HLTF (helicase-like transcription factor) has been shown to bind DNA promoter elements and stimulate transcription in cooperation with Sp1and Sp3 transcription factors.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 625 and 675
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSMARCA3
Antigen Species
Human
Gene ID6596
Gene Symbol
HLTF
Gene Name
helicase like transcription factor
Uniprot IDQ14527
Protein Name
Helicase-like transcription factor
Alternate Names
DNA-binding protein/plasminogen activator inhibitor 1 regulator • DNA-binding protein/plasminogen activator inhibitor-1 regulator • helicase-like transcription factor • HIP116 • HIP116A • HLTF1 • RING finger protein 80 • RING-type E3 ubiquitin transferase HLTF • RNF80 • SMARCA3 • SNF2L3 • SNF2-like 3 • sucrose nonfermenting protein 2-like 3 • sucrose nonfermenting-like 3 • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3 • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 • ZBU1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support.
The epitope recognized by A300-230A maps to a region between residues 600 and 700 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3 using the number given in entry NP_003062.2 (GeneID 6596).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:3000 - 1:10,000
Documents
Product DatasheetPDF · A300-230A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-230A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-230AOpen
Citations / Publications
Services
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