Bethyl Laboratories
Rabbit anti-LIS-1 Antibody Affinity Purified
Primary
Catalog #A300-409A
TargetLIS-1
HostRabbit
ClonalityPolyclonal
ReactivityRat, Mouse, Human
ApplicationWB
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Product Description
Lissencephaly-1 protein (LIS-1) was identified as a gene associated with Miller-Dieker lissencephaly syndrome, an autosomal dominant disorder characterized by microcephaly along with anomalies of the kidney, heart, and gastrointestinal tract. LIS-1 is the non-catalytic alpha subunit of the intracellular isoform of platelet-activating factor acetylhydrolase. LIS-1 plays an important role in neuron migration during brain development and fibroblast migration during wound healing. Its role in migration is related to its function in regulating dynein-mediated microtubule sliding.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Rat, Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLIS-1
Antigen Species
Human
Gene ID5048
Gene Symbol
PAFAH1B1
Gene Name
platelet activating factor acetylhydrolase 1b regulatory subunit 1
Uniprot IDP43034
Protein Name
Platelet-activating factor acetylhydrolase IB subunit alpha
Alternate Names
LIS1 • LIS-1 • LIS2 • lissencephaly 1 protein • Lissencephaly-1 protein • MDCR • MDS • NudF • PAF acetylhydrolase 45 kDa subunit • PAFAH • PAF-AH 45 kDa subunit • PAFAH alpha • PAF-AH alpha • platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) • platelet-activating factor acetylhydrolase IB subunit alpha • platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD) • platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to LIS-1 immobilized on solid support.
The epitope recognized by A300-409A maps to a region between residue 1 and 50 of human Lissencephaly 1 using the numbering given in entry NP_000421.1 (GeneID 5048).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:500 - 1:2500
Documents
Product DatasheetPDF · A300-409A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-409A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-409AOpen
Citations / Publications
Services
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