Bethyl Laboratories
Rabbit anti-TPX2 Antibody Affinity Purified
Primary
Catalog #A300-430A
TargetTPX2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
TPX2 is a microtubule associated protein (MAP) that co-localizes with spindle microtubules during M-phase of the cell cycle. It may function to nucleate microtubules and initiate microtubule polymerization and assembly for spindle formation. TPX2 is a target of RanGTP, a small GTPase that coordinates spindle assembly. TPX2 protein levels peak during mitosis and are tightly regulated by ubiquitination during the cell cycle.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 697 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetTPX2
Antigen Species
Human
Gene ID22974
Gene Symbol
TPX2
Gene Name
TPX2, microtubule nucleation factor
Uniprot IDQ9ULW0
Protein Name
Targeting protein for Xklp2
Alternate Names
C20orf1 • C20orf2 • differentially expressed in cancerous and non-cancerous lung cells 2 • differentially expressed in lung cells • DIL2 • DIL-2 • FLS353 • GD:C20orf1 • HCA519 • HCTP4 • hepatocellular carcinoma-associated antigen 519 • hepatocellular carcinoma-associated antigen 90 • p100 • preferentially expressed in colorectal cancer • protein fls353 • REPP86 • restricted expression proliferation associated protein 100 • Restricted expression proliferation-associated protein 100 • targeting protein for Xklp2 • TPX2, microtubule-associated protein homolog • TPX2, microtubule-associated, homolog
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TPX2 immobilized on solid support.
The epitope recognized by A300-430A maps to a region between residue 700 and the C-terminus (residue 749) of human TPX2 using the numbering given in Swiss-Prot entry Q9ULW0 (GeneID 22974).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:5000 - 1:15,000
Documents
Product DatasheetPDF · A300-430A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-430A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-430AOpen
Citations / Publications
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