Bethyl Laboratories
Rabbit anti-Phospho RPS6 (S235/236) Antibody Affinity Purified
Primary
Catalog #A300-584A
TargetRPS6 Phospho (S235/S236)
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Image 1 of 1
local_shipping
Order within 13 hours and 53 minutes for shipment by today
Product Description
Ribosomal protein S6 (RPS6) is a component of the 40S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPS6 is phosphorylated by many cellular kinases including ribosomal protein S6 kinase that is regulated via the mammalian target of rapamycin (mTOR) pathway. RPS6 has been demonstrated to be important in regulating cell size, glucose homeostasis, and translational control of mRNA.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 235/236
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetRPS6 Phospho (S235/S236)
Antigen Species
Human
Gene ID6194
Gene Symbol
RPS6
Gene Name
ribosomal protein S6
Uniprot IDP62753
Protein Name
40S ribosomal protein S6
Alternate Names
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using a synthetic peptide representing phosphorylation at serine 235 and serine 236 and surrounding residues of RPS6 immobilized on solid support.
Immunogen for A300-584A was a phosphorylated synthetic peptide, which represented a portion of human Ribosomal Protein S6 (GeneID 6194) around serine 235 and serine 236 according to the numbering given in entry NP_001001.2.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2500 - 1:10,000
Documents
Product DatasheetPDF · A300-584A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-584A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-584AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1