Bethyl Laboratories
Rabbit anti-Chk2 Antibody Affinity Purified
Primary
Catalog #A300-619A
TargetChk2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Chk2 functions as a checkpoint protein kinase in response to DNA damage to maintain genomic stability. The yeast homologs are RAD53 and Cds1. The two major upstream controllers of Chk2 are ATM and ATR which are part of the phosphatidylinositol 3-kinase family. When activated, Chk2 targets p53, Brca1, Cdc25A and Cdc25C to cause cell cycle arrest. Chk2 is also known as serine/threonine-protein kinase CHK2, CHEK2, CDS1, LFS2, RAD53, HuCds1, and PP1425.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 493 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetChk2
Antigen Species
Human
Gene ID11200
Gene Symbol
CHEK2
Gene Name
checkpoint kinase 2
Uniprot IDO96017
Protein Name
Serine/threonine-protein kinase Chk2
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Chk2 immobilized on solid support.
The epitope recognized by A300-619A maps to a region between residues 475 and 525 of human Checkpoint Kinase 2 using the numbering given in entry NP_009125.1 (GeneID 11200).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:250 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-619A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-619A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-619AOpen
Citations / Publications
Services
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