Bethyl Laboratories
Rabbit anti-GbetaL Antibody Affinity Purified
Primary
Catalog #A300-680A
TargetGbetaL
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
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Product Description
GbetaL (G-protein beta-subunit-like) is a member of the WD-repeat family of proteins that share a highly conserved tryptophan-aspartate repeating motif that mediates the assembly of multiprotein factors.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 276 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGbetaL
Antigen Species
Human
Gene ID64223
Gene Symbol
MLST8
Gene Name
MTOR associated protein, LST8 homolog
Uniprot IDQ9BVC4
Protein Name
Target of rapamycin complex subunit LST8
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GbetaL immobilized on solid support.
The epitope recognized by A300-680A maps to a region between residues 275 and the C-terminus (residue 326) of human G protein Beta subunit-like using the numbering given in entry NP_071767.2 (GeneID 64223).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-680A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-680A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-680AOpen
Citations / Publications
Services
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