Bethyl Laboratories
Rabbit anti-BRG1/SMARCA4 Antibody Affinity Purified
Primary
Catalog #A300-813A
TargetBRG1/SMARCA4
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. BRG1/SMARCA4 is an ATPase subunit of the SWI/SNF-A chromatin remodeling complex (BAF complex). In BAF complexes, BRG1/SMARCA4 has been shown to interact with BRCA1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetBRG1/SMARCA4
Antigen Species
Human
Gene ID6597
Gene Symbol
SMARCA4
Gene Name
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Uniprot IDP51532
Protein Name
Transcription activator BRG1
Alternate Names
ATP-dependent helicase SMARCA4 • BAF190 • BAF190A • brahma protein-like 1 • BRG1 • BRG1-associated factor 190A • BRM/SWI2-related gene 1 • CSS4 • global transcription activator homologous sequence • homeotic gene regulator • hSNF2b • mitotic growth and transcription activator • MRD16 • nuclear protein GRB1 • protein brahma homolog 1 • protein BRG-1 • RTPS2 • SNF2 • SNF2-beta • SNF2L4 • SNF2LB • SNF2-like 4 • sucrose nonfermenting-like 4 • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 • SWI2 • transcription activator BRG1
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BRG1/SMARCA4 immobilized on solid support.
The epitope recognized by A300-813A maps to a region between residue 75 and 125 of human BRM/SWI2-Related Gene 1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, member 4) using the numbering given in entry NP_003063.2 (GeneID 6597).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-813A · Lot 5 (current lot)Open
Product DatasheetPDF · A300-813A · Lot 5 (current lot)Open
Safety Data SheetPDF · A300-813AOpen
Citations / Publications
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