Bethyl Laboratories
Rabbit anti-ACC1/ACC-alpha Antibody Affinity Purified
Primary
Catalog #A300-987A
TargetACC1/ACC-alpha
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
ACC1/ACC-alpha catalyzes the carboxylation of acetyl-CoA to malonyl-CoA in the enzymatic pathway of long-chain fatty-acid synthesis. ACC1/ACC-alpha has been identified as a partner of BRCA1. Through this interaction, ACC1/ACC-alpha may contribute to the tumor suppressor activity of BRCA1 as demonstrated by the finding that disruption of this interaction increases lipid synthesis in mammary epithelial cells. In this context, lipogenesis has been closely linked to tumorigenesis and ACC1/ACC-alpha has been found to be highly expressed in human breast carcinomas.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2296 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetACC1/ACC-alpha
Antigen Species
Human
Gene ID31
Gene Symbol
ACACA
Gene Name
acetyl-CoA carboxylase alpha
Uniprot IDQ13085
Protein Name
Acetyl-CoA carboxylase 1
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ACC1/ACC-alpha immobilized on solid support.
The epitope recognized by A300-987A maps to a region between residue 2333 and the C-terminus (residue 2383) of human acetyl-Coenzyme A carboxylase alpha using the numbering given in entry NP_942131.1 (GeneID 31).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A300-987A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-987A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-987AOpen
Citations / Publications
Services
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