Bethyl Laboratories
Rabbit anti-SMARCA2/BRM Antibody Affinity Purified
Primary
Catalog #A301-014A
TargetSMARCA2/BRM
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA2/Brm is an ATPase subunit of the SWI/SNF-A chromatin remodeling complex (BAF complex).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSMARCA2/BRM
Antigen Species
Human
Gene ID6595
Gene Symbol
SMARCA2
Gene Name
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Uniprot IDP51531
Protein Name
Probable global transcription activator SNF2L2
Alternate Names
ATP-dependent helicase SMARCA2 • BAF190 • BAF190B • brahma homolog • BRG1-associated factor 190B • BRM • global transcription activator homologous sequence • hBRM • hSNF2a • NCBRS • probable global transcription activator SNF2L2 • protein brahma homolog • SNF2 • SNF2/SWI2-like protein 2 • SNF2-alpha • SNF2L2 • SNF2LA • Sth1p • sucrose nonfermenting 2-like protein 2 • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2 • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 • SWI2
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SMARCA2/BRM immobilized on solid support.
The epitope recognized by A301-014A maps to a region between residue 1 and 50 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 using the numbering given in entry CAA51407.1 (GeneID 6595).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-014A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-014A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-014AOpen
Citations / Publications
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