Bethyl Laboratories
Rabbit anti-SMARCA2/BRM Antibody Affinity Purified
Primary
Catalog #A301-016A
TargetSMARCA2/BRM
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA2/Brm is an ATPase subunit of the SWI/SNF-A chromatin remodeling complex (BAF complex).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1325 and 1375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSMARCA2/BRM
Antigen Species
Human
Gene ID6595
Gene Symbol
SMARCA2
Gene Name
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Uniprot IDP51531
Protein Name
Probable global transcription activator SNF2L2
Alternate Names
ATP-dependent helicase SMARCA2 • BAF190 • BAF190B • brahma homolog • BRG1-associated factor 190B • BRM • global transcription activator homologous sequence • hBRM • hSNF2a • NCBRS • probable global transcription activator SNF2L2 • protein brahma homolog • SNF2 • SNF2/SWI2-like protein 2 • SNF2-alpha • SNF2L2 • SNF2LA • Sth1p • sucrose nonfermenting 2-like protein 2 • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2 • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 • SWI2
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SMARCA2/BRM immobilized on solid support.
The epitope recognized by A301-016A maps to a region between residue 1325 and 1375 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 using the numbering given in entry CAA51407.1 (GeneID 6595).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-016A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-016A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-016AOpen
Citations / Publications
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