Bethyl Laboratories
Rabbit anti-WDR33 Antibody Affinity Purified
Primary
Catalog #A301-151A
TargetWDR33
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 6 hours and 35 minutes for shipment by today
Product Description
WDR33 is a member of the WD repeat protein family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. Studies show that WDR33 is a nuclear protein that is highly expressed in testis, and it is suggested to play a role in cytodifferentiation or DNA recombination.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 975 and 1025
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetWDR33
Antigen Species
Human
Gene ID55339
Gene Symbol
WDR33
Gene Name
WD repeat domain 33
Uniprot IDQ9C0J8
Protein Name
pre-mRNA 3' end processing protein WDR33
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR33 immobilized on solid support.
The epitope recognized by A301-151A maps to a region between residue 950 and 1000 of human WD repeat domain 33 using the numbering given in entry NP_060853.3 (GeneID 55339).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-151A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-151A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-151AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1