Bethyl Laboratories
Rabbit anti-PIBF Antibody Affinity Purified
Primary
Catalog #A301-155A
TargetPIBF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Progesterone-induced blocking factor (PIBF) was originally discovered as a progesterone-induced protein that mediates the immunological effects of progesterone. PIBF is produced in PR-positive lymphocytes in healthy pregnant woman. A decreased percentage of PIBF-positive lymphocytes in pregnant woman is associated with premature pregnancy termination. PIBF appears to exert its immunomodulatory and anti-abortive effects by altering cytokine production and regulating natural killer (NK) cell activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 707 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPIBF
Antigen Species
Human
Gene ID10464
Gene Symbol
PIBF1
Gene Name
progesterone immunomodulatory binding factor 1
Uniprot IDQ8WXW3
Protein Name
Progesterone-induced-blocking factor 1
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PIBF immobilized on solid support.
The epitope recognized by A301-155A maps to a region between residue 685 and 735 of human progesterone-induced-blocking factor 1 using the numbering given in entry NP_006337.2 (GeneID 10464).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-155A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-155A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-155AOpen
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