Bethyl Laboratories
Rabbit anti-RFWD3 Antibody Affinity Purified
Primary
Catalog #A301-396A
TargetRFWD3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
RFWD3 (ring finger and WD repeat domain 3) has been identified as a protein that is phosphorylated in response to DNA damage. The exact function of RFWD3 (ring finger and WD repeat domain 3) has not been characterized. Structurally, RFWD3 possesses a RING-type zinc finger and 3 WD repeats.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRFWD3
Antigen Species
Human
Gene ID55159
Gene Symbol
RFWD3
Gene Name
ring finger and WD repeat domain 3
Uniprot IDQ6PCD5
Protein Name
E3 ubiquitin-protein ligase RFWD3
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RFWD3 immobilized on solid support.
The epitope recognized by A301-396A maps to a region between residue 75 and 125 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-396A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-396A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-396AOpen
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