Bethyl Laboratories
Rabbit anti-AKAP12/Gravin Antibody Affinity Purified
Primary
Catalog #A301-402A
TargetAKAP12/Gravin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
AKAP12/Gravin is a tumor suppressor protein belonging to the A-kinase anchoring protein family. A-kinase anchoring proteins function as molecular scaffolds for the subcellular compartmentalization of A- kinase signaling molecules. In addition to its role in cell signaling, AKAP12/Gravin has also been identified as an autoantigen in some cases of myasthenia gravis and has recently been shown to play a role in leukemogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1732 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAKAP12/Gravin
Antigen Species
Human
Gene ID9590
Gene Symbol
AKAP12
Gene Name
A-kinase anchoring protein 12
Uniprot IDQ02952
Protein Name
A-kinase anchor protein 12
Alternate Names
A kinase (PRKA) anchor protein 12 • AKAP 250 • AKAP-12 • AKAP250 • A-kinase anchor protein 12 • A-kinase anchor protein 250 kDa • A-kinase anchor protein, 250kDa • Gravin • kinase scaffold protein gravin • Myasthenia gravis autoantigen • myasthenia gravis autoantigen gravin • Src-Suppressed C Kinase Substrate • SSeCKS
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AKAP12/Gravin immobilized on solid support.
The epitope recognized by A301-402A maps to a region between residue 1731 and 1781 of human A kinase (PRKA) anchor protein (gravin) 12 using the numbering given in entry AAC51366.1 (GeneID 9590).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-402A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-402A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-402AOpen
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