Bethyl Laboratories
Rabbit anti-PIP1/WDR84 Antibody Affinity Purified
Primary
Catalog #A301-549A
TargetPIP1/WDR84
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
PIP1/WDR84 (PAK1IP1) was identified as a p21-activated protein kinase (PAK) interacting protein that functions as a negative regulator of PAK activity and PAK signaling pathways. PIP1/WDR84 (PAK1IP1) contains 5 G beta-like WD repeats. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPIP1/WDR84
Antigen Species
Human
Gene ID55003
Gene Symbol
PAK1IP1
Gene Name
PAK1 interacting protein 1
Uniprot IDQ9NWT1
Protein Name
p21-activated protein kinase-interacting protein 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PIP1/WDR84 immobilized on solid support.
The epitope recognized by A301-549A maps to a region between residue 290 and 340 of human PAK/PLC-interacting protein 1 (WD repeat protein 84) using the numbering given in entry NP_060376.2 (GeneID 55003).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A301-549A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-549A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-549AOpen
Citations / Publications
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