Bethyl Laboratories
Rabbit anti-PIP1/WDR84 Antibody Affinity Purified
Primary
Catalog #A301-550A
TargetPIP1/WDR84
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
PIP1/WDR84 (PAK1IP1) was identified as a p21-activated protein kinase (PAK) interacting protein that functions as a negative regulator of PAK activity and PAK signaling pathways. PIP1/WDR84 (PAK1IP1) contains 5 G beta-like WD repeats. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 342 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPIP1/WDR84
Antigen Species
Human
Gene ID55003
Gene Symbol
PAK1IP1
Gene Name
PAK1 interacting protein 1
Uniprot IDQ9NWT1
Protein Name
p21-activated protein kinase-interacting protein 1
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PIP1/WDR84 immobilized on solid support.
The epitope recognized by A301-550A maps to a region between residue 342 and 392 of human PAK/PLC-interacting protein 1 (WD repeat protein 84) using the numbering given in entry NP_060376.2 (GeneID 55003).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-550A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-550A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-550AOpen
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