Bethyl Laboratories
Rabbit anti-WDR42A Antibody Affinity Purified
Primary
Catalog #A301-556A
TargetWDR42A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
WDR42A (WD repeat-containing protein 42A) contains seven WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. The function of WDR42A has not been characterized.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetWDR42A
Antigen Species
Human
Gene ID50717
Gene Symbol
DCAF8
Gene Name
DDB1 and CUL4 associated factor 8
Uniprot IDQ5TAQ9
Protein Name
DDB1- and CUL4-associated factor 8
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR42A immobilized on solid support.
The epitope recognized by A301-556A maps to a region between residue 50 and 100 of human WD repeat domain 42A using the numbering given in entry NP_056541.2 (GeneID 50717).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-556A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-556A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-556AOpen
Citations / Publications
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